The Xiamen Symposium on Marine Environmental Sciences

2027 Xiamen Symposium on Marine Environmental Sciences

January 12-15, 2027

Multiple stress factors affecting on the growth and grazing rates of the heterotrophic dinoflagellate species, Oxyrrhis marina
ID:1075 Poster Presentation

2027-01-15 16:00 (China Standard Time)

Session:Session 27 - Priority Contaminants across the Marine Continuum: Behavior, Bioavailability and Ecological Risk Assessment

Abstract
Multiple stress factors affecting on the growth and grazing rates of the heterotrophic dinoflagellate species, Oxyrrhis marina
LI Guoyu, TANG, C.H.
  Dinoflagellates are key marine primary producers whose responses to combined stressors remain limited understanding. Oxyrrhis marina, a cosmopolitan heterotrophic dinoflagellate tolerant of variable coastal conditions, provides a useful model for assessing the interactive effects of polycyclic aromatic hydrocarbons (PAHs) and ultraviolet (UV) radiation. This study aims to quantify how PAHs type and concentration, together with varying UV intensities, influence the growth, survival, and physiology of O. marina. Cultures of O. marina, initially fed Isochrysis galbana, are exposed in f/2 medium to 3 kinds of  representative PAHs (Naphthalene, Phenanthrene, Pyrene) at 0μg L⁻¹, 50μg L⁻¹, 500μg L⁻¹, and 2000 μg L⁻¹ under low, medium, and high UV regimes (UVA: 0.3–2.5 W m⁻²; UVB: 0.1–1.0 W m⁻²). UV levels are manipulated using controlled lamp distance and UV-screening materials. Cells abundance and net growth rates are determined periodically, and prey ingestion rates are estimated using established grazing models. PAHs concentrations in water and algal pellets are quantified by GC/MS. Cellular stress is evaluated by measuring intracellular reactive oxygen species (ROS) via 2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence and antioxidant responses through superoxide dismutase (SOD) activity.
  Based on current experimental results, the control group showed very rapid growth. Using day 3 and day 7 as the time intervals for calculation, the growth rate of Oxyrrhis marina reached 0.5278d-1, the average density of prey algae (Isochrysis galbana,) during the growth period was 76,000 cells/mL, and the feeding rate was 4.3742cells⋅ind-1⋅d-1. In the ongoing multi-factor stress experiment, the results from day 0 and day 3 show that the higher the concentration of naphthalene, the greater the inhibition of Oxyrrhis marina growth; simultaneously, due to the higher level of UV radiation, the growth of both Oxyrrhis marina and Isochrysis galbana, was affected, and the inhibition became stronger with increasing naphthalene concentration. These preliminary experimental results still need to be verified by subsequent experiments over a period of time.

 
Speaker
Guoyu LI Hong Kong Metropolitan University

Author
Guoyu LI Hong Kong Metropolitan University

    Important Dates

    • Jan 30

      2026

      Session / Event proposals open

    • Mar 31

      2026

      Session / Event proposals close
      (Extended to April 7)

    • Apr 30

      2026

      Session acceptance notifications

    • Apr 30

      2026

      Abstract submissions & registration open

    • Jun 30

      2026

      Abstract submissions close
      (Extended to July 21)

    • Aug 31

      2026

      Abstract acceptance notifications

    • Sep 15

      2026

      Scientific program released

    • Oct 15

      2026

      Early bird registration closes

    • Jan 12–15

      2027

      XMAS 2027